Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 17: Posters: Physics of Cells

BP 17.11: Poster

Mittwoch, 28. März 2012, 17:30–19:30, Poster A

A real-time adaptive exposure system for studies of eukaryotic chemotaxis — •Alexander Anielski and Carsten Beta — Biological Physics, Universität Potsdam, Germany

The aim of our project is to quantify the chemotactic motion of eukaryotic cells. In particular, we developed a setup that allows us to separately address the dependencies of the chemotactic motion on the average background concentration and on the gradient steepness. Also, the role of spatial versus temporal sensing can be analyzed with this setup. Our method is based on compensating the cell movement by automated motion of the microscope stage. To achieve this, a software grabs frames from a camera, recognize the cell position, and moves it to the center of the field of view by automatically adapting the position of the microscope stage. To generate a well definied gradient signal, caged compounds in a fluid flow are used in combination with a computer controlled switchable gradient mask (flow photolysis). We exemplify our method with chemotactic cells of the social amoeba Dictyostelium discodideum. The motion and fluorescence data will be used to test competing models of eukaryotic chemotaxis.