Berlin 2012 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Posters: Physics of Cells
BP 17.29: Poster
Wednesday, March 28, 2012, 17:30–19:30, Poster A
Subsurface Imaging Using Atomic Force Acoustic Microscopy at GHz Frequencies — •Matthias Büchsenschütz-Göbeler1, Yuansu Luo1, Shuiqing Hu2, Chanmin Su2, Walter Arnold1,3, and Konrad Samwer1 — 11. Physikalisches Institut, Universität Göttingen — 2Bruker-Nano Inc., Santa Barbara, CA, USA — 3Department of Materials, Saarland University
We describe a technique to image subsurface structures using Atomic Force Acoustic Microscopy operated at 1 GHz. The devices or samples to be imaged are insonified with 1 GHz ultrasonic waves which are amplitude modulated at a fraction or multiple frequency of cantilever contact-resonance [1]. The transmitted signals are demodulated by the nonlinear tip-surface interaction, enabling one to image defects in the device based on their ultrasonic scattering power which is determined by the ultrasonic frequency, the acoustic mismatch between the elastic properties of the host material and the defects, by their geometry, and by diffraction effects. We investigated defect structures in specially prepared samples, in silicon wafers as well as the cytoskeleton in living cells. Concerning the living cells we are interested to understand the contrast mechanism for imaging and the response to different substrate morphologies. Especially the interplay of cellular elasticity, adhesion and motility are brought into focus.
Financial support by the DFG SFB 937 is thankfully acknowledged.
[1] Imaging of Subsurface Structures Using Atomic Force Acoustic Microscopy at GHz Frequencies S. Hu and C. Su, and W. Arnold, J. Applied Phys. 109, 084324 (2011)