Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Posters: Physics of Cells
BP 17.42: Poster
Mittwoch, 28. März 2012, 17:30–19:30, Poster A
Biofilm Formation on Microstructured Components — •Jennifer Marx1, Christine Müller1, Christin Schlegel3, Kai Muffler3, Guido Schüler2, Michael Walk2, Jan C. Aurich2, Roland Ulber3 und Christiane Ziegler1 — 1Department of Physics and Research Center OPTIMAS — 2Institute of Manufacturing Technology and Production Systems — 3Institute of Bioprocess Engineering
As soon as a biological molecule, for example bacteria, gets in contact with a surface, they adsorb to the surface. As result a biofilm is established. Especially in bioreactors the bacteria are used to gain for example pharmaceutical products. In this work micro milled titanium compared to native titanium is used as substrate for the biofilms. The biofilm establishment, the morphology of the bacteria and the adhesion of the cells is investigated by a combination of fluorescence microscopy and scanning force as well as scanning electron microscopy (SFM, SEM) as a reference method. The fluorescence dyes show the genetic material in the bacteria in combination with the extracellular matrix and in addition the discrimination between live and dead cells in the biofilm. An almost living biofilm can be observed on natural titanium. The calibration of optical microscopy and SFM allows the detailed imaging of the cells. On natural titanium a multi-layered biofilm with a fusion of colloidal cells to chains is observed. The SEM approves this observation. and provides an insight into detailed structures of the biofilm. On microstructured titanium a biofilm in the microstructures was observed.