Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: Physics of Cells I
BP 2.12: Vortrag
Montag, 26. März 2012, 12:45–13:00, H 1028
Viscosity-Sensing and Mechano-Transduction of Cells on Adhesive Lipid Bilayers — Lena Astrid Lautscham1, Corey Yu-Hung Lin2, •Daniel Minner2, Wolfgang Goldmann1, Christoph Naumann2, and Ben Fabry1 — 1University Erlangen-Nuremberg, Department of Physics — 2Indiana University-Purdue University, Department of Chemistry and Chemical Biology
Adherent cells have been shown to sense the mechanical properties of their extracellular matrix and to respond to it by altering their morphology, migration speed, and cytoskeletal organization. Previous studies focused on substrate elasticity to investigate cellular mechano sensing and transduction. Here, we altered the substrate viscosity as an alternative route to probe the influence of matrix mechanics on cell responses. Polymer-tethered multi-lipid bilayer systems on a solid support provide a method to tune the substrate viscosity over an extended range by altering the number of stacked bilayers. In contrast to elastic substrates where deformations come to a halt when cell tractions reach a steady state, cell adhesion ligands in viscous substrates remain mobile and thus provide a different mechanical stimulus. To maintain cell tractions, cells need to continuously reorganize their focal adhesions and associated cytoskeletal structures. We probed mechanical bilayer properties as well as cytoskeletal properties with magnetic tweezers. Our data indicate that mouse embryonic fibroblasts (MEF) are extremely susceptible to decreasing substrate viscosity and respond by altering their cytoskeletal and focal adhesion dynamics, cytoskeletal stiffness and spreading area.