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BP: Fachverband Biologische Physik

BP 2: Physics of Cells I

BP 2.3: Vortrag

Montag, 26. März 2012, 10:15–10:30, H 1028

Contractile forces facilitate the enhanced glycosyl-phosphatidylinositol-anchored receptor CD24-dependent invasiveness of cancer cells — •Claudia Tanja Mierke — University of Leipzig, Institute for Experimental Physics I, Soft Matter Physics Division

The malignancy of tumors depends on the capability of cancer cells to metastasize. The process of metastasis involves cell invasion through the extracellular matrix (ECM). Cell invasion is a fundamental biomechanical process, which usually requires adhesion to the ECM through mainly beta1 heterodimeric integrin receptors. The localization of beta1 integrins to lipid rafts depends on the glycosyl-phosphatidylinositol-anchored receptor CD24. The expression of CD24 is up-regulated in several tumor types and consistently associated with increased metastasis in patients. Here, the invasion of A125 lung cancer cells with different CD24 expression levels was studied in 3D-ECMs. The hypothesis was that CD24 expression increases the invasion of cancer cells through increased contractile forces. To analyze this, A125 cells (CD24negative) were stably transfected with CD24 and sorted for high and low CD24 expression. The invasiveness of CD24high and CD24low transfectants were determined in 3D-ECMs. The percentage of invasive cells and their invasion depth were increased in CD24high compared to CD24low cells. Fourier-transform-traction-microscopy revealed that CD24high cells generated 5-fold higher contractile forces compared to CD24low cells. Finally, these results suggest that CD24 enhances cell invasion through increased contractile forces.