Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Posters: DNA/RNA and Related Enzymes
BP 24.13: Poster
Donnerstag, 29. März 2012, 17:30–19:30, Poster A
Nanopore Translocation Dynamics of a Single DNA-Bound Protein — •Andre Spiering1, Sebastian Getfert2, Andy Sischka1, Katja Tönsing1, Karsten Rott3, Peter Reimann2, and Dario Anselmetti1 — 1Experimental Biophysics and Applied Nanoscience, Bielefeld University, 33615 Bielefeld, Germany — 2Soft Matter Theory, Bielefeld University — 3Thin Films and Physics of Nanostructures, Bielefeld University
The single molecule translocation dynamics of various dsDNA-protein complexes upon threading through a solid-state NP was investigated by quantitative 3D-optical tweezers (OT) in the presence of an electric field (NP force spectroscopy). In our single molecule translocation experiments, we find distinct asymmetric force signals that depend on the protein type and charge, the DNA elasticity and its counter-ionic screening in the buffer [1,2]. In order to increase the resolution of these force signals even further we drastically decreased the thickness of the membrane containing the nanopore. We prepared graphene monolayers by exfoliation from graphite, transferred them to Si/Si3N4-support chips and milled the freestanding graphene membrane (with a focused ion beam or a TEM) to produce graphene nanopores of diverse size and shape. Comparing the results for different membranes and nanopores is the basis to further understand the exact physical properties of such translocation dynamics and may lead to the development of novel nanopore sensing and sequencing devices.
[1] A. Sischka et al.: J. Phys.: Condens. Matter 22: 454121 (2010)
[2] A. Spiering et al.; Nano Letters 11: 2978-2982 (2011)