Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Posters: DNA/RNA and Related Enzymes
BP 24.1: Poster
Donnerstag, 29. März 2012, 17:30–19:30, Poster A
Information transfer and readout in complex DNA mixtures — Harish Bokkasam1 and •Christian Trapp2 — 1Institute for Biological Experimental Physics, University of Saarland, Saarbruecken, Germany — 2Institute for Biological Experimental Physics, University of Saarland, Saarbruecken, Germany
Project: Development of an enzyme based method for the copy of oligos with predetermined length from biological template, given knowledge of the therein contained oligonucleotide sequence. In this project, we modify the conventional PCR technique by to generate linearly amplified copies of single stranded oligos. To recover the ssDNA transcripts from the linear PCR reaction, various techniques like gel extraction, column filtration, magnetic beads are used. These purified transcripts are fluorescently labelled in different ways like in-strand labelling and 5/3 end labelling for microarray analysis. To visualize ssDNA fragments of very short length and low concentration here denatured polyacrylamide gel electrophoresis with silver staining is used. From this gel electrophoreses we can approximate the length of the product.
Conclusion: Our method has given very promising results so far. An improvement would be to narrow the length distribution of the transcribed sequences further. Currently we are validating our technique by using a DNA microarray.