Berlin 2012 – scientific programme

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BP: Fachverband Biologische Physik

BP 26: Posters: Membranes and Vesicles

BP 26.20: Poster

Thursday, March 29, 2012, 17:30–19:30, Poster A

Testing for domain formation in ER-mimicking and native ER membrane GUV. — •Mária Hanulová, Gernot Guigas, Julia Hoffmann, and Matthias Weiss — Experimentalphysik 1, Uni Bayreuth, Universitätsstr. 30, 95444 Bayreuth

Proteins that are correctly folded are trafficked from the endoplasmic reticulum (ER) to the Golgi apparatus via COPII vesicles. The vesicles bud off from specialized micrometer-sized membrane domains, so-called ER exit sites, after being coated with COPII proteins. Structurally and functionally distinct domains in cellular membranes such as ER exit sites can be based on lipid immiscibility or induced by binding of proteins. Using fluorescence microscopy, we tested for possible lipid-based domain formation in GUV electroformed from ER-mimicking lipid mixtures and native ER membranes. Native ER membranes were isolated from Hela cells by gradient centrifugation. Both types of GUV were homogeneous and the diffusion coefficients measured by FCS were similar to pure liquid-disordered lipid membranes.