Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 26: Posters: Membranes and Vesicles

BP 26.6: Poster

Donnerstag, 29. März 2012, 17:30–19:30, Poster A

Modeling vesicular exocytosis in chromaffin cells — •Daungruthai Jarukanont¹, Martin Garcia¹, Imelda Bonifas², and Ricardo Femat² — ¹Institut für Physik Universität Kassel — ²División de Matemáticas Aplicadas, IPICyT, San Luis Potosí, Mexico

In cell communications, the transport of vesicles is essential for storage and release of chemical messenger molecules via exocytosis. To understand vesicular exocytosis, current signals induced by transmitter molecules from single cells experiments were measured. Each exocytotic event is characterized by a current spike, and therefore a measurement is represented by a time-series. By performing a series of experiments on chromaffin cells, including cells with pharmacological manipulation of transmitter level with L-DOPA and reserpine, and a careful statistical analysis, we found that the probability for exocytosis events follows a gamma distribution. Combining this with the results from other studies by microscopy method [1], we developed a model for the mechanism of vesicular release and were able to simulate these processes in good agreement with experiment.

[1] Steyer,J. A., Horstmann,H. and Almers,A.,Transport, capture and exocytosis of single synaptic vesicles at active zones, Nature 406, 849-854 (2000)