Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 28: Posters: Imaging
BP 28.1: Poster
Donnerstag, 29. März 2012, 17:30–19:30, Poster A
Morphological analysis of MDCK Epithelial tissue on different substrates — •Sara Kaliman1, Christina Jayachandran2, Ana-Sunčana Smith1, and Florian Rehfeldt2 — 1Institute for Theoretical Physics and Cluster of Excellence: EAM, FA University Erlangen, Germany — 23rd institute of Physics-Biophysics, University of Göttingen, Germany
Significant progress in comprehending the morphology and the internal organization of single cells has been achieved over the last decade. However, cell agglomerates and tissues are understood to a much lesser extent. Here we study the positioning and the orientations of the cell nuclei with respect to the cell body, as well as the organization and the correlations between the cells in two-dimensional epithelial tissues. For this purpose, epithelial Madin-Darby canine kidney (MDCK) cells are grown on collagen coated polyacrylamide gels of various elastic moduli (1-34 kPa) and on stiff glass substrates. Upon fixation and double staining, all cell-lines were imaged in fluorescence, the results of which were processed by a self-made MATLAB program. We find a similar organization in tissues with equivalent local density, irrespective of the substrate elasticity, even though the dynamics of the tissue growth depends on the underlaying matrix. In all cases, we find little deviations from random distributions. Furthermore, favorable comparison between true cell body and the Voronoi construction based on the nu- clei shape suggests that the intracellular interactions dominate those of cells with the substrate. Consequently, the morphology of cells in tissues differ significantly from that of individual cells.