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BP: Fachverband Biologische Physik

BP 28: Posters: Imaging

BP 28.3: Poster

Donnerstag, 29. März 2012, 17:30–19:30, Poster A

3-Dimensional Characterization of Fibroblasts with Enhanced Differential Interference Contrast Microscopy — •Malte Ohmstede, Erik Bernitt, and Hans-Günther Döbereiner — Institut für Biophysik, Universität Bremen

Circular dorsal-ruffles, a phenomenon found on the dorsal surface of fibroblast cells, are not yet well understood, actin based membrane protrusions. Algorithms for characterization of three-dimensional properties and dynamics have been developed for evaluation of optical sectioning data, which is gained with differential interference contrast. Due to the low optical density of fibroblast specimens, differential interference contrast is used. Optical sectioning data allows for evaluation of living specimens, thus enabling characterization of dorsal ruffle dynamics. Differential interference contrast, however, complicates evaluation of image data due to gradient-like image properties. Complications with image segmentation, i.e. separating image foreground and background, have been resolved by application of a two-dimensional Hilbert Transform on image data to gain bright-field-like images from gradient sources. Further processing was done by executing morphological filters and skeletonization on the binary images gained by segmentation. Contour data gained by skeletonization was then extracted for further evaluation and processing.