Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 28: Posters: Imaging

BP 28.4: Poster

Donnerstag, 29. März 2012, 17:30–19:30, Poster A

Measuring the local refractive index of cells with a laser trap based interferometer — •Kai Bodensiek, Paula Sánchez, Yvonne Kretzer, and Iwan Schaap — III Physikalisches Institut, Faculty of Physics, Georg-August Universität, Götingen, Germany

To be able to measure the spatial distribution of the refractive index in individual living cells we constructed a micrometer sized Fabry-Pérot interferometer that was placed in a microscope sample chamber. One mirror of the interferometer is formed by the microscope coverslip and the second movable mirror was positioned with a laser trap in a direction perpendicular to the coverslip. The local cellular refractive index is extracted by simultaneously measuring the local height of the cell and the cell induced phase shift of the interference signal. Because the instrument is implemented in a diffraction-limited optical microscope we can study the local organization of the cell and its nucleus.