Berlin 2012 – scientific programme
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BP: Fachverband Biologische Physik
BP 28: Posters: Imaging
BP 28.6: Poster
Thursday, March 29, 2012, 17:30–19:30, Poster A
Nanotomography of bovine tendon — •Stefan Kubitza1, Stephanie Röper1, Anke Bernstein2, and Robert Magerle1 — 1Chemische Physik, TU Chemnitz, D-09107 Chemnitz — 2Department für Orthopädie und Unfallchirugie, Muskuloskelettales Forschungslabor, Universitätsklinikum Freiburg, 79106 Freiburg
Collagen is the most abundant fibrous protein in mammals. It can be found in various types of biological tissue, for example in tooth, bone and tendon. We use atomic force microscopy (AFM) based nanotomography for imaging the nanoscaled structure of this biological material. The specimen is ablated layer-by-layer by wet chemical etching and imaged with tapping mode AFM after each etching step. From resulting series of AFM images the three-dimensional structure is reconstructed. In our experiments we focus on native bovine tendon (collagen type I) and report about the suitability of different etching solutions for nanotomography imaging.