Berlin 2012 – scientific programme

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BP: Fachverband Biologische Physik

BP 28: Posters: Imaging

BP 28.8: Poster

Thursday, March 29, 2012, 17:30–19:30, Poster A

Multifocus Fluorescence Correlation Spectroscopy using a programmable phase modulator — •Thomas Kuckert, Zdeněk Petrášek, and Petra Schwille — Biotechnologisches Zentrum, TU Dresden, Tatzberg 47/49, 01307 Dresden, Germany

Fluorescence Correlation Spectroscopy (FCS) has been used for studying molecular dynamics and interactions in many fields, ranging from physics, chemistry to biology. Many applications, especially those within living cells or tissues where the measurement time is limited, would benefit from the ability to perform measurements at different positions simultaneously. This requires both excitation localized within a number of well-defined volumes, and separate detection of fluorescence from within these volumes. Here we present an ongoing project the goal of which is to construct such a flexible multifocus FCS setup. Previously, multiple excitation spots have been realized by diffractive optical elements designed specifically for a given spot pattern, without the possibility to adapt the pattern to the sample. We use a programmable phase modulator (PPP), which allows flexible formation of a variable number of foci at arbitrary positions. Initially, avalanche photodiodes will be used for detection, to assess the quality of foci produced by the PPP. Eventually, fast EM-CCD is intended for simultaneous detection from all foci to reach full flexibility.