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BP: Fachverband Biologische Physik

BP 31: Imaging

BP 31.1: Topical Talk

Freitag, 30. März 2012, 09:30–10:00, H 1058

High-speed imaging of organogenesis in entire zebrafish with SPIM — •Jan Huisken — Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

In the past the recording speed of a time-lapse experiment has ultimately been limited by the amount of light the specimen could tolerate. Lately, it has been shown that light sheet microscopy such as Selective Plane Illumination Microscopy (SPIM) reduces photo-toxic effects to a minimum. Due to the illumination of the sample in a thin volume around the focal plane no tissue outside the plane of interest is exposed and bleached. In addition, the fluorescence is collected with very high sensitivity cameras. SPIM benefits from the latest camera technology and is therefore constantly improving in speed and sensitivity.

Experiments have become possible that run at full speed using the best possible hardware, without being limited by the fragility of the sample. The speed advantage of the SPIM over other fluorescence technique can be utilized to image rapid events in developing tissue or to record a large number of views for multi-view reconstruction. The large amount of data that is accumulated when modern cameras are run at high-speed for hours or days is enormous and innovative data processing solutions are needed. One key application of this emerging technology includes the multi-dimensional imaging of the developing zebrafish larvae over extended periods of time. I will give some examples of the unique capabilities of SPIM, especially for monitoring the development of the cardiovascular system and the early endoderm.

M. Weber and J. Huisken, Curr Opin Genet Dev, 21, 566-72 (2011).