Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 31: Imaging

BP 31.4: Vortrag

Freitag, 30. März 2012, 10:30–10:45, H 1058

Multimodal imaging of the human cerebellum: phase contrast tomography, magnetic resonance microscopy and histology — •Georg Schulz¹, Timm Weitkamp², Irene Zanette^3,4, Franz Pfeiffer⁴, Conny Waschkies⁵, Christian David⁶, and Bert Müller¹ — ¹BMC, Uni Basel, Switzerland — ²Synchrotron Soleil, Gif sur Yvette, France — ³ESRF, Grenoble, France — ⁴Biomedical Physics, TUM, Garching, Germany — ⁵IBT, ETH / Uni Zurich, Switzerland — ⁶LMN, PSI, Villigen, Switzerland

To visualize a part of the human cerebellum we use grating based phase contrast CT, MR microscopy and histology. Phase contrast tomography yields the 3D distribution of the X-ray refractive index and is much more sensitive than conventional absorption imaging. Using a grating interferometer we are able to detect deflection angle differences of around 20 nrad. The CT results were acquired at ESRF Grenoble using a 5 µm pixel size detector. The high sensitivity of the method even provides contrast between structures within the gray matter. The MR microscopy data set was acquired using a T2*-weighted 3D FLASH sequence with an isotropic pixel length of 45 µm. The method allows a clear differentiation between white and gray matter but shows marginal contrast between the two structures within the gray matter. During histology a Nissl staining was applied. While the micrographs have a high spatial resolution of less than a µm and a contrast comparable to the X-ray phase contrast data, they do not provide 3D information. The complementarity of X-ray phase-contrast CT, MR microscopy and histology gives better insights into the morphology of the human brain.