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BP: Fachverband Biologische Physik

BP 32: Physics of Cells III

BP 32.3: Vortrag

Freitag, 30. März 2012, 10:00–10:15, H 1028

Quantification of Depletion Induced Adhesion of Red Blood Cells — •Patrick Steffen¹, Claude Verdier², and Christian Wagner¹ — ¹Universität des Saarlandes, Sarbrücken, Germany — ²CNRS - Universite de Grenoble I, Laboratoire Interdisciplinaire de Physique

Under physiological conditions, red blood cells are known to form aggregates in the forms of rouleaux due to the presence of plasma proteins. Roleaux formation can be also induced in vitro by the adddition of macromolecules to washed red blood cells. Current data on the adhesion strength between red blood cells in their natural discocyte shapes are limited. Here we present measurements on the dextran induced aggregation of red blood cells by use of atomic force microscopy based single cell force spectroscopy (SCFS). The effects of dextran concentration and molecular weight on the interaction energy of adhering RBCs was determined. The results are in excellent agreement with a model based on the depletion effect and former experimental studies.