Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 32: Physics of Cells III
BP 32.4: Vortrag
Freitag, 30. März 2012, 10:15–10:30, H 1028
‘Wound Healing in vitro’: Blood Platelets on Structured Substrates — •Rabea Sandmann1, Sarah Schwarz G. Henriques1, Florian Rehfeldt2, and Sarah Köster1 — 1Institute for X-Ray Physics & CRC Physics, University of Göttingen, Germany — 2Third Institute of Physics, University of Göttingen, Germany
Blood platelets are anuclear, adherent cells primarily responsible for blood clotting and their activation can be triggered by, e.g., soluble factors like thrombin. Whenever a wound arises, the endothelial cell layer inside blood vessels is disrupted and underlying proteins become exposed, which creates a micro- and nanostructured surface. It is therefore important to understand how platelets react to micro- and nanostructured surfaces. Our results show that isotropic structures in the µm-range do not have an influence on the orientation of cells or the ordering of the stress fibers. However, the degree of surface coating determines the size of the platelets. Furthermore, platelets build distinct geometrical shapes on unstructured glass. Quite similar shapes can be observed on microstructured PDMS substrates. Therefore, the process of shape formation may be governed by underlying nanostructures. We examine the effect of such nanostructured substrates, which have the potential to alter the ordering of stress fibers by confinement of the focal adhesions’ positions. Our experiments contribute to the fundamental understanding of cell behavior in general, and may have direct applications in medicine due to the importance of platelets in wound healing.