Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 32: Physics of Cells III

BP 32.7: Vortrag

Freitag, 30. März 2012, 11:00–11:15, H 1028

Direct observation of catch-bonds in focal adhesions of living cells — •Navid Bonakdar, Achim Schilling, Claus Metzner, Michael Kuhn, Richard Gerum, and Ben Fabry — Biophysics Group, University of Erlangen, Germany

Single molecule force spectroscopy data have demonstrated that the chemical bonds between extracellular matrix proteins, integrins, and several proteins of the focal adhesion complex show catch-bond behavior: the binding strength increases under mechanical load. It remains unknown, however, whether catch-bond mechanisms are of any relevance for stabilizing matrix adhesions in living cells. To measure adhesion strength, we bind RGD-coated magnetic beads to integrin adhesion receptors of living cells and apply forces of up to 80 nN with a magnetic tweezer. In the case of a pulling force that increases linearly with time, the characteristic bead detachment force is expected to increase logarithmically with the loading rate for thermally activated Bell-type molecular bonds. We find that the detachment force tends to increase faster than logarithmically, demonstrating that the adhesion bonds strengthen under force. This may be indicative of catch bonds, but could also arise from a complex binding energy landscape. To distinguish between these two possibilities, we applied a staircase-like mechanical load with the same average loading rate but with forces that at all times exceeded those of the linear ramp protocol. We find significantly increased detachment forces under a staircase-like loading protocol compared to a linear force ramp, which rules out other mechanisms except catch-bond behavior.