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BP: Fachverband Biologische Physik

BP 7: Posters: Proteins

BP 7.2: Poster

Montag, 26. März 2012, 17:30–19:30, Poster A

Characterization and Application of the redox-sensitive GFP-mutant roGFP — •Sebastien Peter¹, Sebastian Wierer³, Kirstin Elgass⁴, Stefan Bieker¹, Alfred Meixner², Ulrike Zentgraf¹, and Frank Schleifenbaum¹ — ¹Zentrum für Molekularbiologie der Pflanzen, Universität Tübingen, Deutschland — ²Institut für physikalische und theoretische Chemie, Universität Tübingen, Deutschland — ³Protein Crystallography and Molecular Bioinformatics, Universität Konstanz, Deutschland — ⁴Biochemistry Department, La Trobe University, Bundoora, Australia

For the quantitative analysis of molecular processes in living (plant) cells new combinatorial approaches in optical and spectroscopic technologies are required. The use of green fluorescent protein (GFP) and its variants has revolutionized the in vivo analysis of cell biological processes. More recently, fluorescent proteins have been designed and adapted for targeted sensing of cellular metabolites. Recent progress has been made in generating a redox- sensitive mutant of GFP (roGFP). Here, we characterize the optical properties of roGFP in dependence on the environmental redox state including a comprehensive comparison of different spectral analysis approaches in vitro. Furthermore, we demonstrate several applications of roGFP in living plants such as long-term redox potential readout in plant cells.