Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters: Proteins
BP 7.40: Poster
Montag, 26. März 2012, 17:30–19:30, Poster A
Biological applications for nano-mechanical detection of molecular recognition — •Andreas Mader1, Kathrin Gruber1, Roberto Castelli2, Peter H. Seeberger2, Joachim O. Rädler1, and Madeleine Leisner1 — 1LMU München, Fakultät fuer Physik — 2Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin
Advances in carbohydrate sequencing technologies have revealed the tremendous complexity of the glycome. Understanding the biological function of carbohydrates requires the identification and quantification of carbohydrate interactions with biomolecules. The increasing importance of carbohydrate-based sensors able to specifically detect sugar binding molecules or cells, has been shown for medical diagnostics and drug screening. Our biosensor, with a self-assembled mannoside sensing layer, specifically detects carbohydrate-protein binding interactions (mannoside - ConA), as well as real time interaction of carbohydrates with different E. coli strains in solution. Binding to the cantilever surface causes mechanical surface stress, that is transduced into a mechanical force and cantilever bending. The degree and duration of cantilever deflection correlates with the interaction`s strength. In this study we present carbohydrate-based cantilever biosensors as a robust, label-free, and scalable method to analyze carbohydrate-protein and carbohydrate-bacteria interactions. The cantilevers thereby exhibit specific and reproducible deflection with a high sensitivity range of over four orders of magnitude.