Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 7: Posters: Proteins

BP 7.41: Poster

Montag, 26. März 2012, 17:30–19:30, Poster A

Dynamic force spectroscopy on fluorescence labeled tau-peptides and monoclonal antibodies measured by using Optical Tweezers — •Tim Stangner, Carolin Wagner, David Singer, Christof Gutsche, Olaf Ueberschär, Ralf Hoffmann, and Frierich Kremer — Uni Leipzig, Leipzig, Germany

Since humans become older and older with the fast evolution of medicine, degenerative diseases edge ever closer to focus of research. Especially Alzheimer's disease is the most common form of dementia. Each Alzheimer patient shows two commonly known changes in the brain: senile plaques of β-amyloid-peptide and tangles of hyper-phosphorylated tau proteins. Dynamic force spectroscopy (DFS) is performed by using optical tweezers on the level of single receptor-ligand-interactions. Here we report about the specific binding of two anti-human tau-monoclonal antibodies (mAbs), HPT-104 and HPT-110, interacting with synthetic (non-)fluorescence-labeled tau-peptides with different phosphorylation pattern. The fluorescent tagged tau-peptides, anchored on Melanmin-resin beads, are presorted with the fluorescence activated cell sorting (FACS) method in order to achieve homogenous surface coverage. Specific binding events between peptide and mAbs are described according to the Dudko-Hummer-Szabo-model [1]. A comparison between labeled and non-labeled tau-peptide and their interactions with mAbs shall show the influence of the linker- (PEG-spacer) and the fluorescein-molecule on the parameters, obtained by the Dudko-Hummer-Szabo-model [1].

References: [1] Dudko et al.; PNAS 2008 vol. 105 no. 41 15755-15760