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BP: Fachverband Biologische Physik

BP 7: Posters: Proteins

BP 7.5: Poster

Montag, 26. März 2012, 17:30–19:30, Poster A

(contribution withdrawn) Dynamic disorder in enzyme catalyzed reactions: a general phenomenon? — Tatyana Terentyeva¹, Hans Engelkamp², Alan E. Rowan², Tamiki Komatsuzaki³, Johan Hofkens¹, Chun-Biu Li³, and •Kerstin Blank² — ¹Katholieke Universiteit Leuven, Department of Chemistry, Leuven, Belgium — ²Radboud University Nijmegen, Institute for Molecules and Materials, Nijmegen, The Netherlands — ³Hokkaido University, Research Institute for Electronic Science, Sapporo, Japan

Single molecule fluorescence experiments allow the recording of the sequence of individual enzymatic turnover reactions. Experiments with different enzymes have shown that the waiting times between turnovers follow a stretched exponential distribution interpreted as dynamic disorder. Although easily explained with the existence of several enzyme conformations with different activity the question remains if dynamic disorder is a general property of enzymes. Studying the enzyme chymotrypsin we observe deviations from a stretched exponential distribution. Moreover, we see obvious differences in the shape of the waiting time distributions depending on the data analysis method used. Comparing the performance of the most widely employed binning and thresholding approach with change point analysis we observe that the underlying on- and off-histograms are not necessarily extracted from the "signal" buried in noise. When using the more accurate change point analysis for data of chymotrypsin no characteristics of dynamic disorder can be found. In light of these new results, dynamic disorder might not be a general characteristic of enzymatic reactions.