Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Posters: Biopolymers and Biomaterials (with CPP)
BP 8.2: Poster
Montag, 26. März 2012, 17:30–19:30, Poster A
A Dielectrophoresis Study on the Frequency-Dependent Behaviour of Sepsis Pathogens in Media with Different Conductivities — •Ulrich-Christian Schröder1,2, Uwe Glaser1,2, Christian Leiterer2, Andrea Csâki2, Wolfgang Fritzsche2, Uwe Hübner2, Jürgen Popp2,3, Michael Bauer1, and Ute Neugebauer1,2 — 1Center for Sepsis Control and Care, Jena University Hospital — 2Institute of Photonic Technology Jena e.V. — 3Institute for Physical Chemistry and Abbe Center of Photonics, University Jena
Sepsis is a heavy inflammatory response of the human body due to the invasion of pathogenic organisms, which leads to high mortality rates. To ensure a start of early therapy, we are developing a highly sensitive, rapid, label-free and culture independent Lab on Chip method to estimate the resistance of sepsis pathogens towards antibiotics. Dielectrophoresis makes use of the interaction of high frequency non-uniform electrical fields with dielectric nano- and microparticles and can be used to manipulate sepsis pathogens within microsized regions. In the presented work the dielectrophoretic behaviour of the sepsis pathogens is studied for different frequency ranges as well as for different medium conductivities to trap and collect these organisms for further non-invasive optical characterization and furthermore to use dielectrophoresis as a diagnostic tool to distinguish between living and dead bacteria. Acknowledgement: The financial support of the BMBF (FKZ 01EO1002) is highly acknowledged.