Berlin 2012 – scientific programme

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BP: Fachverband Biologische Physik

BP 8: Posters: Biopolymers and Biomaterials (with CPP)

BP 8.5: Poster

Monday, March 26, 2012, 17:30–19:30, Poster A

Systematic investigation of lipids on a titanium layer on the incubation time in water and in growth medium via x-ray reflectometry — •Anahita Shahin¹, Dieter Lott¹, Regine Willumeit¹, Bérengère Luthringer¹, Alexander Grünwald², and Andreas Schreyer¹ — ¹Helmholtz-Zentrum Geesthacht, Max-Planck-Str. 1, 21502 Geesthacht — ²II. Physikalisches Institut, Universität zu Köln, Zülpicher Straße 77, 50937 Köln

Soft matter is increasingly investigated with x-ray scattering during the last years for the detailed structural characterization of this important class of samples. In particular it provides a valuable contribution to the area of medical physics, for instance for the optimization of implants. In this work in-situ x-ray reflectometry and diffraction under small angles is used to analyze the structural properties of lipid bilayers under a variety of conditions. Particular attention is paid to a solid-supported membrane system in which a 70 nm titanium layer deposited on a silicon substrate is coated by lipids. The main objective is to analyze how many lipid bilayers can be attached to the solid titanium layer and how the number of layers changes upon the incubation time in growth medium (Dulbecco's Modified Eagle's Medium (DMEM)) and in water. Here we concentrate on time-dependent measurements to find the optimal incubation time for the lipids in the different media. The combination of experiments and simulation of the scattering data is a valuable tool to understand the ordering and time dependence.