Berlin 2012 – scientific programme
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BP: Fachverband Biologische Physik
BP 8: Posters: Biopolymers and Biomaterials (with CPP)
BP 8.9: Poster
Monday, March 26, 2012, 17:30–19:30, Poster A
Characterization of Protein Films on Dental Materials — •Fabian Kratz1, Nils Körber1, Christine Müller1, Natalia Umanskaya2, Matthias Hannig2, and Christiane Ziegler1 — 1Department of Physics and Research Centre OPTIMAS; University of Kaiserslautern; D-676632 — 2Clinic of Operative Dentistry, Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, D-66424
In dentistry a greater knowledge of protein film establishment processes is of high concern, because protein films are the basis for bacterial adhesion. Especially in the human mouth caries is a consequence of a biofilm. The ambition is to characterize protein films on model materials and continuative on dental relevant materials like titanium, gold, natural enamel, PMMA and PTFE under the influence of dental relevant parameters (like pH and the roughness of the material) by using different surface science methods in combination with biochemical assays. The thickness of the protein films, measured by ellipsometry and scanning force microscopy (SFM), corresponds to the size of the protein molecules. This hypothesizes a monolayer adsorption process.. The combination with biochemical methods like BCA assay and enzymatic activity determines the adsorbed amount of protein and its activity in the case of lysozyme. An influence of the surface roughness, the pH value and the surface material on the adsorbed amount is observed by BCA assay. As a function of the surface material and the pH (thus carious conditions) the enzymatic activity differs.