Berlin 2012 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 23: Poster: Polymer Dynamics

CPP 23.13: Poster

Mittwoch, 28. März 2012, 11:00–13:00, Poster A

Two - point Nanorheology based on single molecule FRET in polymer PMA — •subhasis adhikari, markus selmke, and frank cichos — Molecular Nano-photonics, Inst. of Expt. Phys I, University of Leipzig

Fluorescence Resonance Energy Transfer (FRET) has been widely used in structural biology, biochemistry and in polymer science to measure the distances in very short range (2nm - 10nm) depending on the donor - acceptor pair. To study the heterogeneous dynamics in polymers near its glass transition temperature (Tg) a new technique, Two-point Nanorheology based on FRET is used in single molecule optical studies. This study will reveal the rheological information of the local environment surrounding the dye molecules at a nanometer length scale by measuring distance fluctuations. Firstly Alexa 488 (donor) and then Alexa 594 (acceptor) are attached to amino - thiol terminated polystyrene polymer by a standard synthesis and characterized by fluorescence methods.The single donor dye labeled polymer aggregates in DMF but not in toluene as detected by Fluorescence Correlation Spectroscopy (FCS) technique. Ensemble FRET and single molecule FRET (smFRET) are measured and compared to each other. The reorientation factor is calculated from fluorescent anisotropy studies.