Berlin 2012 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 25: Poster: Stress Relaxation in Polymers - From single molecules to biological cells (joint focus with BP)

CPP 25.9: Poster

Mittwoch, 28. März 2012, 11:00–13:00, Poster A

Modeling the stretch and relaxation properties of cytoskeletal filament systems in neutrophil — •Othmar Marti¹, Michael Beil², and Stephan Paschke³ — ¹Institute of Experimental Physics, Ulm University, D-89081 Ulm — ²Department of Medicine, University Hospital Ulm, D-89081 Ulm — ³Department of Surgery, University Hospital Ulm, D-89081 Ulm

The individual contribution of cytoskeletal filament systems to the regulation of cell deformation remains to be elucidated in detail. We investigated the mechanical behavior of differentiated NB4 cells which are a model for neutrophil granulocytes [1]. Cellular deformability was studied by optical stretching [2] before and after cells were exposed to cytochalasine D and colchicine to disassemble the actin cytoskeleton and microtubules, respectively. The mechanical response of the cells in the stretcher was modeled by a mechanical model consisting of parallel and series connections of elastic and viscous elements. We found that the salient features of the deformation curves can be described adequately. Microtubules were discovered to mainly contribute to cell elasticity, whereas filamentous actin was in control of cell viscosity in this model system. This link between the cytoskeleton and cell mechanics might point to novel options for the pharmacological modulation of the cell migration.

[1] Bruel, A., Paschke, S., Jainta, S., Zhang, Y., Vassy, J., Rigaut, J.P. and Beil, M., Anticancer. Res. 21, 3973-80 (2001) [2] Guck, J., Ananthakrishnan, R., Mahmood, H., Moon, T.J., Cunningham, C.C. and Käs, J. , Biophys. J. 81, 767-84 (2001).