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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 26: Poster: New Perspectives of Scattering at Soft Matter

CPP 26.2: Poster

Mittwoch, 28. März 2012, 11:00–13:00, Poster A

Diffusion of proteins in a bicontinuous microemulsions: A combined fluorescence correlation spectroscopy and neutron spin echo study — •Ralph Neubauer, Sebastian Höhn, Christoph Schulreich, and Thomas Hellweg — Phys. und Biophys. Chemie (PC III), Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany

In bicontinuous microemulsions (ME) the oil and water components form sponge-like domains which are separated by a surfactant/co-surfactant film. This structure shows two dynamics: Bulk diffusion (water and oil domain) and film diffusion (surfactant), which can be observed by dynamic light scattering (DLS), neutron spin echo (NSE) and fluorescence correlation spectroscopy (FCS).

For FCS the label determines the type of diffusion which will be observed. Using an amphiphilic fluorescent molecule the diffusion of the surfactant film can be measured. By choosing a fluorescent apolar protein the movement of this particle in the water domain is detected and the confinement effect can be identified. In NSE experiments the deuteration decides about what is measured. If one component in the ME is protonated and the remaining components are matched out by deuteration, only the diffusion of the protonated component is analyzed. When all four components are deuterated and a protein (is inserted in the water phase, the diffusion of the protein will be obtained, also leading to a indepent information about the confinement effect in a ME.