Berlin 2012 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 7: Poster: Biopolymers and Biomaterials (jointly with BP)
CPP 7.2: Poster
Montag, 26. März 2012, 17:30–19:30, Poster A
Diffusion and Adsorption of Proteins in Nanoscaled Environments — •Sebastian Mörz1, Dagmar Auerbach2, Gregor Jung2, and Patrick Huber1,3 — 1Experimental Physics, Saarland University, Saarbrücken, Germany — 2Biophysical Chemistry, Saarland University, Saarbrücken, Germany — 3Facultad de Física, Pontificia Universidad Católica de Chile, Santiago de Chile, Chile
During the recent years, some versatile developements in biochemistry have opened a wide field of applications. Examples are pore-suspending lipid bilayers or new means of drug delivery by encapsulation of biomolecules into mesoporous template materials. In nanometer scaled confinement, both the transport of biomolecules and their interaction with the porous environment are crucial to these applications, since the above mentioned systems employ artificial nanoporous matrices.
The aim of our research is to examine the diffusion and adsorption characteristics of proteins under such confinement in cylindrical nanochannels with special respect to the differences between the folded and the unfolded state of the proteins. Adsorption isotherms of folded and unfolded bovine heart cytochrome c in nanoporous silica have been measured at different pH-values, ranging from near-neutral pH to the isoelectric points of both the protein and the matrix. The diffusion of green fluorescent protein (GFP) in porous aluminum oxide was investigated using fluorescence correlation spectroscopy (FCS) for various pore diameters (25 to 75 nm) at pH 7 and at the isoelectric point of the membrane material (pH 9).