Berlin 2012 – scientific programme

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O: Fachverband Oberflächenphysik

O 13: Polymeric biomolecular films

O 13.7: Talk

Monday, March 26, 2012, 17:30–17:45, MA 005

Structure-property relation in oligoethylene glycol terminated alkanethiol monolayers under electron irradiation as applied to specific and non-specific protein adsorption — •Yekkoni L. Jeyachandran and Michael Zharnikov — Angewandte Physikalische Chemie, Universität Heidelberg, 69120 Heidelberg, Germany

We investigated the possibility to use electron irradiation for the controlled modification of the protein-repelling and protein-affinity properties of oligoethylene glycol (OEG) terminated alkanethiol (AT) monolayers. The modifications were induced either directly by electron irradiation or in combination with the successive exchange reaction with the molecular substituents bearing special anchor group for protein adsorption. The direct irradiation treatment allowed for controlled non-specific adsorption of proteins, whereas the irradiation-promoted exchange reaction (IPER) enabled their controlled specific adsorption. Combining these two approaches with lithography, we fabricated both non-specific and specific protein patterns of various shapes, including gradient ones, on different length scales. The specific protein adsorption relied on well-known biotin-avidin interaction, utilising biotinylated-OEG-AT molecules as the substituents in IPER. Potential applications of gradient protein patterns for cell adhesion and mobility analyses, as well as novel strategies for multi-protein patterning by a combination of direct writing and IPER will be discussed.