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Hannover 2013 – wissenschaftliches Programm

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MO: Fachverband Molekülphysik

MO 14: Poster 1: Cluster, Femtosecond Spectroscopy

MO 14.8: Poster

Dienstag, 19. März 2013, 16:00–18:30, Empore Lichthof

Ultrafast mid-infrared spectroscopy of sensory rhodopsin II from Natronomonas pharaonis — •Christian Kijatkin, Kristin Springfeld, Johann Klare, Heinz-Jürgen Steinhoff, and Mirco Imlau — School of Physics, Osnabrück University, Germany

A multitude of several rhodopsins offer functional properties, which are necessary for the use as pumps, sensors or even molecular phototaxis [1]. Transient mid-infrared vibrational spectroscopy is performed to optain more information of the light-induced trans-cis isomerization process of sensory rhodopsin II from Natronomonas pharaonis (NpSRII). We use femtosecond laser pulses with wavenumbers in the fingerprint region of the chromophore NpSRII between 1180 cm−1 and 1206 cm−1 and of the etyhlenic stretch vibrations between 1530 cm−1 and 1572 cm−1.

The transmission of mid-infrared τ≈150 fs pulses is detected by a multichannel MCT detector as a function of time delay to an intense VIS pump pulse of τ≈100 fs pulse duration. We present our results on the studies of the light-induced changes of such vibrational modes on the 100 fs time scale compared to FTIR difference spectra.

Financial support by the DFG (IM 37/5, INST 190/137-1) is gratefully acknowledged.

[1] J. P. Klare et al., Bioenergetics, 45, 73-122 (2008)

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