Hannover 2013 – scientific programme
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MO: Fachverband Molekülphysik
MO 19: Poster 2: Biomolecules, Cold Molecules, Experimental Techniques, Various Topics
MO 19.25: Poster
Wednesday, March 20, 2013, 16:00–18:30, Empore Lichthof
Single-particle orbit tracking: A new method for tracking fluorescent particles with high temporal and spatial resolution. — Dominic Raithel1, Daniel Zalami1, Dominique Ernst1, Markus Retsch2, •Gerken Uwe1, and Jürgen Köhler1 — 1Experimental Physics IV, University of Bayreuth, 954440 Bayreuth, Germany — 2Physical Chemistry I, University of Bayreuth, 954440 Bayreuth, Germany
Diffusion and transport processes play an important role in many fields of natural and material sciences. Such processes were generally studied with fluorescence-correlation spectroscopy (FCS), a technique based on confocal microscopy, as well as wide-field fluorescence microscopy using CCD cameras. We demonstrate a new technique in the field of single-particle tracking, lately proposed by J. Enderlein [1], called single-particle orbit tracking (SPOT). With SPOT a single fluorescent particle is traced with the focus of the excitation laser rotating on a circle which encloses the particle. With this technique we are able to follow a fluorescent polymer bead for more than 10 min with a temporal resolution of 4 ms and position accuracy in the range of several nanometers [2].
[1] J. Enderlein, Appl. Phys. B (71), 773-777 (2000) [2] D. Ernst et al., JOSA (29), 1277-1287 (2012)