Hannover 2013 – scientific programme

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Q: Fachverband Quantenoptik und Photonik

Q 43: Poster II

Q 43.25: Poster

Wednesday, March 20, 2013, 16:00–18:30, Empore Lichthof

3D super resolution upconversion microscopy of praseodymium-doped yttrium aluminum garnet — •Tugrul Inal, Rainer Stöhr, Roman Kolesov, Rolf Reuter, and Jörg Wrachtrup — 3. Physikalisches Institut and Stuttgart Research Center of Photonic Engineering (SCoPE), Universität Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany

Super resolution fluorescence microscopy has caught great attention due to providing an optical resolution beyond the Abbe diffraction limit. Several diffraction unlimited techniques such as stimulated emission depletion (STED) and ground state depletion (GSD) microscopy have been developed for that purpose [1].

In previous work, we have demonstrated super-resolution upconversion microscopy of praseodymium-doped yttrium aluminum garnet (Pr:YAG) nanoparticles.This technique provides background-free imaging due to ultraviolet upconverted fluorescence of Pr:YAG nanoparticles [2].Combining the upconversion properties with a super-resolution optical microscopy technique similar to STED, yields to a lateral resolution of approximately 40 nm which is of the order of the size of the particles under study.

In this contribution, we discuss an additional improvement to the technique by increasing also the axial resolution far beyond the diffraction limit. Using the appropriate phase retardation plates, a resolution of approximately 40 nm in all three dimensions is achieved.

[1] Hell, S. W. et.al. Science 316, 1153-1158(2007) [2] Kolesov, R. et al. Phys. Rev. B 84, 153413 (2011)