Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Posters: Imaging

BP 10.11: Poster

Montag, 11. März 2013, 17:30–19:30, Poster B2

Broadening the applications of vibrational spectroscopy in microscopy with super resolution techniques — •Christian Pilger, Henning Hachmeister, Michael Stührenberg, Lena Nolte, Gerd Wiebusch, and Thomas Huser — Biomolecular Photonics, Universität Bielefeld, Germany

In vibrational spectroscopy coherent anti-Stokes Raman scattering (CARS) is an established method for imaging biological samples on the microscopic scale. This process results in the specific excitation of molecular bonds. Utilizing a four-wave mixing process the Raman signal is strongly enhanced, and can now be used e.g. for imaging inner cell structures.

In comparison to fluorescence microscopy, CARS requires no organic fluorophores to highlight cellular structures of interest. Thereby the biological structures and processes are unaltered and the signal is persistent.

This type of laser scanning microscopy is, however, still limited in its spatial resolution by the diffraction limit. We are exploring a number of potential ways that will enable us to overcome the diffraction limit in nonlinear optical microscopy. Ultimately, we hope to be able to image individual viral particles in vivo during their entire life cycle from the assembly of new virions to the infection of new cells.