Regensburg 2013 – scientific programme

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BP: Fachverband Biologische Physik

BP 10: Posters: Imaging

BP 10.16: Poster

Monday, March 11, 2013, 17:30–19:30, Poster B2

Raman spectroscopic characterization of sepsis relevant bacteria appearing in urinary tract infections on a dielectrophoresis chip — •U.-Ch. Schröder^{1, 2}, C. Assmann¹, A. Ramoji¹, U. Glaser^{1, 2}, U. Hübner², Ch. Leiterer², A. Csáki², W. Fritzsche², M. Bauer¹, J. Popp^{1, 2, 3}, and U. Neugebauer^{1, 2} — ¹Center for Sepsis Control and Care, Jena University Hospital — ²Institute of Photonic Technology Jena e.V. — ³Institute for Physical Chemistry and Abbe Center of Photonics, University Jena

Sepsis reflects a dysregulated response of the immune system due to the invasion of pathogens characterized by high mortality rates. Due to the requirement for overnight cultivation steps the standard microbiological diagnostic methods are too slow for an early start of tailored therapy. To make things worse bacteria are getting more and more resistant towards antibiotics. We have combined direct dielectrophoretic capturing of bacteria in dilute suspensions above a quadrupol microelectrode array with using micro-Raman spectroscopy to achieve a novel rapid, highly specific, label-free and culture-independent lab on chip method. As a proof-of-principle study two commonly participating bacterial strains in urinary tract infections, Escherichia coli and Enterococcus faecalis, are respectively captured, and are classified with respect to their molecular signature. To demonstrate rapid antibiotic susceptibility testing, the differentiation of antibiotic treated and non-treated Enterococcus faecalis strains has been carried out. With the help of multivariate statistical analysis a robust classification model has been established. Acknowledgement: BMBF (FKZ 01EO1002)