Regensburg 2013 – scientific programme
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BP: Fachverband Biologische Physik
BP 10: Posters: Imaging
BP 10.17: Poster
Monday, March 11, 2013, 17:30–19:30, Poster B2
Characterization of Caco-2 cells via confocal Rama Microscopy — •Susanne Kimeswenger1,2, Krisztina Vincze-Minya1, Mohammad-Reza Lornejad-Schäfer2, Klaus Schröder2, and Sabine Hild1 — 1Institute of Polymer Science, Johannes Kepler University, Altenbergerstr. 69, 4040 Linz, Austria — 2Biomed-zet Life Science GmbH, Industriezeile 36/I, 4020 Linz, Austria
Eucaryotic cells consist of 60-70 % water, the rest is bioorganic material, which is highly heterogenous: lipids, peptides, carbohydrates and nucleic acids. The distribution of these components varies in different cell types and cell states. Over the past few years Raman spectroscopy got more and more interesting for the characterization of cells, as Raman spectroscopy is a non-destructive, non-contact in vitro method to analyze cells providing information about their viability, differentiation status and tumorigenicity. The human carcinoma cell line Caco-2 has the special characteristic to showing a small intestine enterocyte phenotype when cultured for 21 days. Caco-2 cells seeded on different substrates (silicium, calcium fluoride) were tested. Confocal Raman microscopy reveals the differences between vital and fixated cells. As correlation of Raman spectra and different cell components in fixated cells was not possible, characterization of Caco-2 cells was performed in vital cell status; the identification of Raman spectra of cell components was promising. Furthermore we have revealed differences between differentiated and proliferated Caco-2 cells regarding to their Raman spectra, reflecting differences in bio-molecular composition.