Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Posters: Imaging

BP 10.2: Poster

Montag, 11. März 2013, 17:30–19:30, Poster B2

Monitoring early embryogenesis with single-plane illumination microscopy — •Philipp Struntz, Rolf Fickentscher, and Matthias Weiss — Experimental Physics I, University of Bayreuth, Germany

Single-plane illumination microscopy (SPIM) is a fluorescence-imaging technique that combines rapid widefield detection with optical sectioning. Due to a reduced phototoxicity induced by bleaching, SPIM is capable of providing a long-term, three-dimensional time-resolved in vivo imaging of specimen. We have built and automated a SPIM-setup specifically designed for imaging eggs of the nematode Caenorhabditis elegans in the early stages of embryogenesis. With the obtained time-lapse images it is possible to track cell positions within the developing embryo to get information about the spatiotemporal development of tissues. Based on this, we have designed a simple model that is capable of explaining experimental observations during the first stage of embryogenesis.