Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Posters: Imaging

BP 10.7: Poster

Montag, 11. März 2013, 17:30–19:30, Poster B2

Wide-field magnetometry imaging using nitrogen-vacancy centers — Steffen Steinert¹, •Nicolas Götz¹, Florestan Ziem¹, Andrea Zappe¹, Liam Hall², Lloyd Hollenberg², and Jörg Wrachtrup¹ — ¹3 Physikalisches Institut, Universität Stuttgart, 70569 Stuttgart Germany — ²2School of Physics, University of Melbourne, Victoria 3010, Australia

Imaging of weak electromagnetic signals with sub-cellular resolution under life sustaining conditions is of great importance in the context of life science, but could not be achieved yet. A diamond doped with a shallow layer of nitrogen-vacancy centers (NV) potentially fulfills this requirements. The spin state of a single negatively charged NV center can be optically polarized and read out. Measuring the local Zeeman shift and spin state lifetime of several NVs enables to determine the magnetic fields in the vicinity of the Diamond. Using a wide-field microscopy technique, we can simultaneously capture the signal of a 60*m x 60*m area of the diamond surface on a CCD camera with diffraction limited resolution. Here we present the investigation of different magnetic species as marker for magnetic cell imaging via microfluidic detection. We also evaluate the feasibility of label-free imaging using this technique.