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BP: Fachverband Biologische Physik

BP 13: Imaging

BP 13.1: Topical Talk

Dienstag, 12. März 2013, 09:30–10:00, H44

Ultrasensitive detection, microscopy, tracking, and manipulation of nano-objects — •Vahid Sandoghdar — Max Planck Institute for the Science of Light, Erlangen, Germany

The advent of fluorescence microscopy and spectroscopy in the 1990s ushered in single molecule detection as a powerful tool for a wide range of studies, ranging from biophysics to quantum optics. Since then a number of techniques have pushed the limits of spatial resolution and detection sensitivity for the visualization of matter down to the single molecule level. In our laboratories, we have approached these issues in two different ways. First, we have developed extinction detection and spectroscopy for investigating nonfluorescent single nano-objects such as metallic nanoparticles, viruses, quantum dots, and organic molecules [1-6]. In particular, I will present measurements of single virus motion and its interaction with receptor lipids [4], studies of nanoparticle fast motion on artificial membranes, and very recent detection of unlabeled single proteins [7]. In the second approach, we exploit cryogenic single molecule detection to push localization microscopy to the angstrom precision level [8].

[1] K. Lindfors, et al, Phys. Rev. Lett. 93, 037401 (2004). [2] P. Kukura, et al, Nano Lett. 9, 926 (2009). [3] P. Kukura, et al, Nature Methods 6, 923 (2009). [4] P. Kukura, et al, J. Phys. Chem. Lett. 1, 3323 (2010). [5] M. Celebrano, et al, Nature Photonics 5, 95 (2011). [6] M. Krishnan, et al, Nature 467, 692 (2010). [7] M. Piliarik and V. Sandoghdar, in preparation. [8] S. Weisenburger, et al, in preparation.