Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 13: Imaging
BP 13.3: Vortrag
Dienstag, 12. März 2013, 10:15–10:30, H44
STED Microscope with Spiral Phase Contrast — •Marcel Andreas Lauterbach1, Marc Guillon1, Asma Soltani2, and Valentina Emiliani1 — 1University Paris Descartes, Paris Sorbonne Cité, Neurophysiology and New Microscopies Laboratory, Paris, France — 2University Paris Descartes, Paris Sorbonne Cité, Laboratory of Membrane Dynamics and Neurological Diseases, Paris, France
We present a STED (STimulated Emission Depletion) microscope with phase contrast imaging capabilities. Phase contrast permits the visualization of weak phase objects without any labeling. It is implemented without extra optical elements in the STED light path, which would compromise STED imaging capabilities. Scanning phase contrast allows for registration with the fluorescence images and principally for simultaneous recording of phase contrast and STED images.
Phase contrast images of objects as thin as a few nanometers in optical path mismatch are obtained. The method provides a second imaging channel in a STED microscope for phase contrast imaging even in index-matched mounted cell cultures. This allows for correlation of morphological structures with high-resolution fluorescence images.