Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Biomaterials and Biopolymers I (joint with CPP)
BP 14.3: Vortrag
Dienstag, 12. März 2013, 10:15–10:30, H34
Single-Virus Force Measurements on sialic acid — •Valentin Reiter1, Sumati Bhatia3, Manuel Gensler1, Christian Sieben2, Daniel Lauster2, Andreas Herrmann2, Rainer Haag3, and Jürgen P. Rabe1 — 1Department of Physics, Humboldt-Universität zu Berlin — 2Department of Biology, Humboldt-Universität zu Berlin — 3Department of Chemistry, Freie Universität Berlin
We study the multivalent effects in the binding and dissociation processes of sialic acid with the viral membrane protein hemagglutinin using scanning force microscopy (SFM) based single-virus force measurements (SVFM). Since an infectious disease has to start with the attachment of a virus to the cell membrane, this binding as well as its dissociation has to be carefully analyzed [1]. This is done by creating a mixed self assembled monolayer (MSAM) on gold from synthesized compounds, terminated by lipoic acid. The compounds are functionalized with sialic acid and disfunctionalized with hydroxide. Inactivated influenza viruses are attached to a silicon nitrate SFM cantilever via a polyethylene glycol spacer. With this setup SVFM is performed in aqueous solution. The ratio of functionalized to disfunctionalized molecules on the MSAM enables the control of the degree of multivalency of the binding and therefore the realization of mono-, bi-, and trivalent connections. On the other hand SVFM are performed at different loading rates to estimate specific binding parameters, such as the average rupture length and bond life time.
C. Sieben et al., PNAS, 2012, 109, 34, 13626-13631.