Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 17: Biomaterials and Biopolymers II (joint with CPP)
BP 17.2: Vortrag
Mittwoch, 13. März 2013, 10:00–10:15, H43
Nano-confined protein anchors, structured by STED lithography, probed by dSTORM. — •Richard Wollhofen1, Moritz Wiesbauer1,2, Kurt Schilcher2, Jaroslaw Jacak1,2, and Thomas A. Klar1 — 1Johannes Kepler University, Linz, Austria — 2Upper Austria University of Applied Sciences, Linz, Austria
The ability to place individual proteins onto nano-confined structures plays a constantly growing role in bioscience, from basic studies in biology to development of nanosensors. One of the possibilities to generate sub-micrometer sized structures is direct laser writing (DLW) lithography. The resolution of DLW can be enhanced by stimulated emission depletion (STED) for assembly of polymeric structures down to several tens of nanometers [1]. Using a pulsed 780nm laser for two-photon DLW and a 532nm laser for STED, we are able to obtain structure sizes of down to 55nm and manufacture two clearly separated lines with 120nm distance [2]. The structures show good biocompatibility and allow an easy biofunctionalization with proteins down to the single protein level. We use direct stochastic optical reconstruction microscopy (dSTORM), which enables determination of protein density at a nanoscale level [3]. Combining STED lithography with dSTORM allows us to produce and characterize biocompatible structures, applicable to many biological assays. [1]J. Fischer et al.,Adv.Mat.,Vol.22,Nr.32,pp.3578-3582(2010); [2]R.Wollhofen et al.,submitted; [3]S. van de Linde et al.,Photochem.&Photobiol.Sc.,Vol.8,Nr.4,pp.465-469(2009);