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BP: Fachverband Biologische Physik
BP 24: Posters: Physics of Cells
BP 24.12: Poster
Mittwoch, 13. März 2013, 17:30–19:30, Poster C
Increased Stiffness of Neutrophils after Activation by Transfusion Related Acute Lung Injury-Relavant Antibodies — •Michael Glaubitz1, Tom Berthold2, Christiane A. Helm3, Mihaela Delcea1, and Andreas Greinacher2 — 1ZIK HIKE - Centre for Humoral Immune Reactions in Cardiovascular Diseases, University of Greifswald — 2Department of Transfusion Medicine, University of Greifswald — 3Department of Physics, University of Greifswald
Transfusion related acute lung injury (TRALI) is a severe adverse effect of blood transfusion. A subgroup of TRALI is induced by antibodies directed against alloantigens on neutrophils, a subgroup of the granulocytes. TRALI is believed to occur in approximately one in every 5000 transfusions. Besides neutrophil aggregation, the neutrophils elasticity could be critical for the development of an acute lung injury, as stiffer neutrophils might get stuck in the narrow microvasculature of the lung. Using colloidal probe or tippless Atomic Force Microscopy (AFM) cantilevers to compress the cells, the influence of TRALI-relevant antibodies (HNA-3a) on the stiffness (Young's modulus E) of neutrophils is investigated. The AFM indentation measurements are fitted to the Hertz model. The stiffness of neutrophils increased after incubation with HNA-3a. The parameter incubation time was investigated and found to be relevant. These findings give insights in the etiology of TRALI.