Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 24: Posters: Physics of Cells

BP 24.30: Poster

Mittwoch, 13. März 2013, 17:30–19:30, Poster C

Automated Optical Stretching — •Roland Stange, Tobias Kießling, Anatol Fritsch, Susanne Rönicke, and Josef Käs — Institut für Experimentalphysik 1, Leipzig, Deutschland

The mechanical behavior of single eukaryotic cells is known to play a defining role in cell migration, cell division, mechanotransduction, tissue formation and embryogenesis. Thus huge effort was made to develop methods able to test single cell mechanics (e.g.: Optical Stretcher, optical tweezer, atomic force microscope, micropipette aspiration, magnetic beat rheology). Despite the low throughput of these methods it got clear, that cells of the same cell type (e.g. from the same tissue or cell-line) are not mechanically equal, but show a broad, non-Gaussian, asymmetric distribution. To further investigate cell mechanics from a statistical perspective we increased the throughput of the Optical Stretcher technique to 300 cells per hour leading to cell counts of more than 1000 cells for a simple measurement. By measuring fully automated, human bias is drastically reduced and resulting distributions are smooth and reliable due to standard errors smaller than 5%.