Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Posters: Physics of Cells
BP 24.5: Poster
Mittwoch, 13. März 2013, 17:30–19:30, Poster C
Collective cell migration in tumor colonies — •Janina Lange1, Claus Metzner1, Julian Steinwachs1, Patrick Krauss1, Pamela Strissel2, and Ben Fabry1 — 1University of Erlangen-Nuremberg, Department of Physics, Biophysics Group — 2University of Erlangen-Nuremberg, Department of Gynaecology and Obstetrics
Many tumor cells proliferate despite a lack of interaction with the extracellular matrix and without cell-contact inhibition that normally prevents cells from proliferating beyond confluency. This gives tumor cells the advantage to grow into a dense 3-dimensional tissue. As the tumor grows, mechanical stresses arise that depend on proliferation speed, cell contractility, substrate adhesiveness, and cell cohesiveness. They are organized by cells migrating between regions of different mechanical stresses. Here we study how proliferation, adhesiveness and cohesiveness influence the migration of individual tumor cells in rapidly growing 3-dimensional tumor colonies initiated on a 2D substrate. Colonies of highly and weakly adhesive and cohesive cell lines are compared. We also study colonies of embryonic mouse fibroblasts in which focal adhesion kinase was knocked out, which leads to changes in both adhesiveness and cohesiveness involving E-cadherin. In weakly adhesive cell lines, cells close to the border migrate rapidly and persistently in the radial direction. Interestingly, in the central tumor region we also find highly persistent cell migration but in random directions with a spatially and temporally highly correlated migration pattern. Collective cell migration, however, was absent in colonies of high adhesiveness and low cohesiveness.