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BP: Fachverband Biologische Physik

BP 25: Posters: Cytoskeleton

BP 25.14: Poster

Mittwoch, 13. März 2013, 17:30–19:30, Poster C

Conception and construction of a microfluidic chamber for the optical tweezers — •Samuel Vollmer, Tobias Paust, and Othmar Marti — Institute of Experimental Physics - University Ulm, D-89069 Ulm, Germany

Microfluidics is the science and engineering of systems in which fluid behavior differs from conventional flow theory primarily due to the small length scale of the system. For many measurement systems small length scales are obligatory for the ability to work with small reagent volumes.

It is important to understand the assembly process of Keratin 8/18 networks and their mechanical properties at early times. Therefore we constructed a microfluidic chamber to mix assembly buffer and dialysis buffer at the optical tweezers location to investigate the network mechanics at starting times.

To find the best geometry for our chamber we first have done some simulations using the finite element method. These simulations show the mixing of the two buffers in dependence of the geometry of the chamber.