Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 25: Posters: Cytoskeleton
BP 25.7: Poster
Mittwoch, 13. März 2013, 17:30–19:30, Poster C
Actomyosin Detection at Fluorescence Interference Contrast Checkpoints for Automated Biocomputation Readout — •Mercy Lard1, Lasse ten Siethoff2, Malin Persson2, Alf Månsson2, and Heiner Linke1 — 1The Nanometer Structure Consortium (nmC@LU) and Division of Solid State Physics, Lund University, Lund, Sweden — 2School of Natural Sciences, Linnaeus University, Kalmar, Sweden
On-chip biotechnologies, which aim to replace microfluidic, driven flow with active molecular-motor driven transport of cytoskeletal filaments include: bio-computation, diagnostics, and drug screening. These applications would benefit greatly from further miniaturization and increased sensitivity. Here we make use of actomyosin in an in vitro motility assay, incorporated in nanostructures, as a platform for bio-simulation of the time evolution of motile objects in complex networks, for example in biocomputation. This type of application can require detection of filaments at checkpoints in the device with high signal-to-noise ratio, for example, to record the number and speed of filaments at a specific location. To serve this need, we make use of fluorescence interference contrast (FLIC) at thin gold lines running perpendicular to nano-sized polymer resist channels that guide filament motion. Using cross-correlation at pairs of Au lines, we strongly enhance S/N and counting accuracy. We demonstrate the tracking of single or multiple filaments at these checkpoints, and discuss limits of this technique. Application areas of this technique will be discussed.