Regensburg 2013 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 26: Posters: Biomaterials and Biopolymers (joint with CPP)

BP 26.5: Poster

Mittwoch, 13. März 2013, 17:30–19:30, Poster C

Temperature treatment of protein layers at the solid/liquid interface in different environments - An x-ray reflectivity study — •Irena Kiesel, Michael Paulus, Julia Nase, Sebastian Tiemeyer, Christian Sternemann, and Metin Tolan — Fakultät Physik / DELTA, Technische Universität Dortmund, 44221 Dortmund, Germany

Proteins at solid/liquid interfaces play a key role in technical, biomedical, and food processing applications. The interaction of bacteria and cells with surfaces is influenced by adsorbed proteins. These proteins can lose their functionality when denatured by e.g. heat treatment.

In our experiment we have investigated in situ the denaturation process of different model proteins (lysozyme, RNase A and BSA) induced by increasing temperature (up to 80°C) in two different environments, a pure buffer solution and a protein solution, which represents a protein reservoir. The measurements were performed using the 27 keV x-ray reflectivity setup at beamline BL9 of the synchrotron radiation source DELTA (Dortmund, Germany). The electron density profiles of the layer system and the amount of the adsorbed proteins were obtained by analysing the reflectivities at each temperature.

We observe that in pure buffer solution the proteins desorb with increasing temperature, whereas temperature treatment of protein layers in protein solution results in thicker protein layers and can be explained by additional protein adsorption.

Work was supported by BMBF (05K10PEC) and NRW Forschungs- schule.