Regensburg 2013 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 27: Biotechnology and Bioengineering
BP 27.4: Vortrag
Donnerstag, 14. März 2013, 10:30–10:45, H43
Biological applications for nano-mechanical detection of molecular recognition — •Andreas Mader1, Kathrin Gruber1, Roberto Castelli2, Peter Seeberger2, Joachim Rädler1, and Madeleine Leisner1 — 1LMU München, Fakultät für Physik — 2Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin
Advances in carbohydrate sequencing technologies have revealed the tremendous complexity of the glycome. Understanding the biological function of carbohydrates requires the identification and quantification of carbohydrate interactions with biomolecules. The increasing importance of carbohydrate-based sensors able to specifically detect sugar binding molecules or cells, has been shown for medical diagnostics and drug screening. Our biosensor with a self-assembled mannoside based sensing layer that specifically detects carbohydrate-protein binding interactions (mannoside - ConA), as well as real time interaction of carbohydrates with different E.coli strains in solution. Binding on the Cantilever surface causes mechanical surface stress, that is transduced into a mechanical force and cantilever bending. The degree and duration of cantilever deflection correlates with the interaction's strength. During this study we could establish that carbohydrate-based cantilever biosensing is a robust, label-free, and scalable method to analyze carbohydrate-protein and carbohydrate-bacteria interactions [1]. The cantilevers thereby exhibit specific and reproducible deflection with a high sensitivity range of over four orders of magnitude.
[1] A.Mader et al. NanoLetters, 2012, 12 (1), pp 420-423